Empirical Modeling of Mechanical Properties of Modified Collagen/Chitosan Membrane by Response Surface Methodology

In this study, collagen/chitosan membrane used for guided bone regeneration (GBR) was modified. Collagen and chitosan are routinely used in GBR membrane fabrication. In addition advanced platelet rich fibrin (A-PRF) is a promising substitution for fabricating membrane in dental surgery. Herein, acid soluble collagen from calf skin was extracted and characterized. The combination of A-PRF with collagen/chitosan membrane was investigated in this study. FTIR analysis revealed that chemical crosslinking using EDC/NHS was occurred. The morphology of collagen/chitosan membrane in a gradient manner of chitosan was assessed via SEM images. Response surface methodology (RSM) was used to quantitatively study the effect of parameters, including chitosan/collagen weight ratio (chit/col) and A-PRF concentration on the mechanical behavior of the fabricated membrane. Results showed that Young's modulus of the prepared membrane increased by increasing and decreasing of chit/col and A-PRF concentration, respectively.